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Air Microflora

For medical students2 min readUpdated 2026-10-10

Air microflora has no permanent native inhabitants; all microorganisms enter the atmosphere from external sources. Aeromicrobiology studies this transient microbial community, its composition, and control methods.

OriginSecondary — microbes enter from soil, water, and the surfaces of living organisms.
Main enemySolar insolation is the primary factor causing microorganism death in the atmosphere.
ConcentrationThe maximum count of bacteria and viruses is always recorded in large urban areas.
SterilityUV irradiation and filtered ventilation are critical for surgical suites.

Origin and Species Diversity

The air environment contains no nutrients and is unfavorable for microorganism reproduction. For this reason, air microflora is exclusively secondary in nature. Bacteria, fungi, and viruses do not live here permanently; instead, they are temporarily transported by wind currents, dust, or liquid droplets. The main sources of atmospheric contamination include soil, open bodies of water, and the skin and mucous membranes of humans and animals.

Despite their temporary (transient) status, the morphological diversity of the aerial environment is extremely high. Practically all life forms of the microbial world can be found in air samples. These include various cocci, non-spore-forming rods, bacilli, clostridia, actinomycetes, as well as microscopic fungi and viruses.

Survival Factors and Geographic Distribution

The viability of microbial cells in the open atmosphere is severely limited. The main physical factor causing the mass destruction of microorganisms in the air is direct solar insolation. Natural ultraviolet radiation destroys cellular structures, preventing the global spread of many infections.

Microbial distribution shows a distinct geographic pattern:

Characteristics of Indoor Microflora

Inside buildings, a specific ecological environment is formed. The quantitative and qualitative composition of indoor microflora directly depends on the maintained sanitary regime. Key parameters determining air purity include the frequency of cleaning, ventilation intensity, natural lighting levels, and, most importantly, human crowd density. The more people in an enclosed space, the higher the concentration of microorganisms.

Sanitation and Air Purification Methods

To reduce the microbial load in medical, laboratory, and public facilities, a strict set of sanitation methods is applied. The basic and mandatory method is regular wet cleaning, which prevents the resuspension and circulation of bacteria-bearing dust.

Specialized sanitation methods include:

Mnemonic

To easily remember places with a minimum concentration of microbes, think of the ideal vacation route far from people: forests, mountains, and seas.

Frequently asked questions

Which sanitary-indicator bacteria are studied when assessing indoor air purity?

When assessing indoor air purity, researchers test for bacteria that belong to the human upper respiratory tract microflora. Key sanitary-indicator microorganisms include:

  • Golden staphylococcus (Staphylococcus aureus) — a target microorganism whose presence is strictly regulated (e.g., it must be completely absent in operating rooms).
  • Hemolytic streptococci — indicators pointing to a common pathway of airborne pathogen transmission.

Staphylococci in general, as well as pathogenic (coagulase-positive) staphylococci, may also be studied.

What methods and devices are used for air sampling in microbiological research?

Sedimentation, aspiration, and filtration methods are used for microbiological air sampling.

  • Sedimentation method — free settling of microbes onto open Petri dishes with nutrient agar for 5 minutes; calculations are performed using Omeliansky's formula.
  • Aspiration method — forced deposition of microorganisms via air jet impaction onto agar. Devices used include the Krotov apparatus, Typhoon-R40M sampler, Bio Sas Super, and Mas 100 instruments.
  • Filtration method — drawing air through a liquid medium using Rechmensky, POV-1, or Dyakonov devices.
What phases are bacterial aerosols typically divided into based on particle size and settling velocity?

Depending on particle size, microbial aerosols are divided into three main phases.

  • Coarse-droplet liquid phase — consists of droplets over 0.1 mm (>100 µm) in diameter, representing a water-salt shell around bacteria. Their airborne lifespan is several seconds.
  • Fine-droplet liquid phase — includes droplets smaller than 0.1 mm.
  • Bacterial dust — the third phase of bacterial aerosol.
Why is air microflora considered secondary?

Air is not a natural habitat for microbial reproduction. Microbes enter it temporarily from primary reservoirs—soil and water—or shed from human and animal surfaces.

What is the main factor causing bacterial death in the atmosphere?

The primary damaging factor in the open environment is direct solar insolation, which has a lethal effect on most microorganisms.

What determines indoor air purity?

It is determined by the established sanitary regime: regularity of wet cleaning, ventilation frequency, lighting levels, and the density of people occupying the space.

What sanitation methods are mandatory for operating rooms?

Continuous filtered air ventilation and regular UV irradiation with germicidal lamps are critical for operating rooms and microbiological laboratories.

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